Isagi Yoichi, a first-year forward, loses his chance for the Nationals because he has passed the ball to his fellow player in front of the goal. That's when he receives an invitation for Blue Lock. It's a facility to train one world's greatest striker out of 300 high-school-aged forwards so that Japan can win the World Cup.

The first selection is the round-robin tournament of five teams of V, W, X, Y and Z in Building Five, with only top two teams being eligible to move forward. Isagi's Team Z, the lowest ranking team, plays against Team X. But because of the special rule that allows the top scorer of the team to proceed to the next section, the match turns into chaotic scrambling for the ball, regardless of the teams. It was Barou Shoei, Team X's formidable striker, who changes the flow of the game.

Isagi relies on "the smell of the goal," his raw instincts, to make a move, and it leads to the fierce battle of "reading" each other's hand with Team Y's playmaker, Niko Ikki. Isagi and Niko's battle as they both watch over the whole field and throw their ego at each other, brings them to the last one minute of the match. Can Isagi and Team Z beat Niko's final strategy, "One Time Kill Counter"?

After the match with Team Y, Isagi's rank moved up, and he became the center of the team. The next match is against Team W, led by the Wanima twins. Their eye contact and impeccable combinations are hard to beat. They used to play in the same team as Chigiri Hyoma, and they use his past trauma to egg him on. Chigiri says that he joined Blue Lock to find a reason to quit soccer. Team Z leads the game with Kuon's play. Everyone's elated for the good start of the match, but Isagi feels something is off.

Rin chooses to add Isagi to his team, and together with Bachira they pass the second selection. Waiting for them on the other side is a 5-on-5 match against a team of world-class players. Thanks to Isagi, Bachira, and Rin's coordinated play, they manage to score the first goal, but they soon find themselves overwhelmed by the opposing team's superior skill and ability. Despite this, they desperately keep fighting until the last second, unleashing their own "egos." As a result, the "Blue Lock" project, aiming to produce the world's greatest striker, enters a new phase.