It's been a week since the resolution of the crossroad killing case, in which the name of the Kamiya Kasshin-ryu was deceptively used. Although the name of Kamiya Dojo has been cleared, there's no sign of an increase in the number of Kaoru's students. Then a passing boy, Yahiko Myojin, pickpockets Kenshin's wallet. Kenshin gives him the wallet, saying that it was his fault for allowing it to get stolen. Yahiko is repulsed at being treated like a child, but he has a reason to collect the money.

Kenshin receives a request from the police chief, asking for his help to take down "Kurogasa," a serial killer who has been repeatedly targeting former Revolutionary warriors. Kenshin and Sanosuke go to escort Jusanro Tani, who received a letter containing a death threat. As warned in the letter, at 1:00 a.m. sharp, Kurogasa appears. His true identity was Jine Udo, a vagabond slayer who had made a name for himself in the assassination business since the Bakumatsu Era.

In order to kill Kenshin, Kanryu uses his Gatling gun and rains a flurry of bullets at him, uncaring that the Oniwaban Group that was under his command would also be hit by this attack. In the face of this evil act, the Oniwaban Group make full use of their individual abilities to counter the Gatling gun. At this moment, theirs and Kenshin's purpose become one. Kenshin and the Oniwaban group team up to challenge the overwhelming power of a modern weapon.