The Ushiromiya family gathers on Rokkenjima, a small island off the coast of Japan, for their annual family conference to discuss how the inheritance of the ailing family head, Kinzo, will be distributed among his children and their spouses. Battler, who has not attended the conference in six years, notices a new painting in the foyer of the mansion. He is told that it is a portrait of the "Golden Witch" Beatrice, who had supposedly given Kinzo all his wealth, and who Kinzo desires to see again. Battler, however, does not believe in witches or magic, and doubts she exists. A typhoon approaches the island, and Maria is left outside by her mother Rosa while searching for a wilted rose George had marked for her in the garden. When it begins to rain, Battler, Jessica, George, and Rosa go outside to find Maria with an umbrella she did not have before, which Maria says Beatrice gave her.

Rosa recounts how she met the human Beatrice confined in a hidden mansion, Kuwadorian, on the island twenty years ago and helped her escape, only to see her accidentally fall off a cliff to her death. Beatrice confirms to Battler that she truly died as a human, and that there are currently no more than 18 people on the island, forcing Battler to accept his loved ones as possible culprits. Genji, Shannon, and Kanon are later summoned to Kinzo's study, where they see Beatrice offer him as a sacrifice and kill him. Kanon is pit against the seven Sisters of Purgatory, defeating Lucifer in a duel, though he and Shannon are overwhelmed as they try to fend them all off at once, prompting Genji to euthanize Shannon and Kanon before he is put to rest by Ronove. The Sisters search the mansion for more sacrifices, killing Gohda in the process. They find Kumasawa as well, who claims to have been waiting for Beatrice. Beatrice reveals Kumasawa to be her predecessor and teacher, the former Beatrice.

In 1998, Ange arrives at Rokkenjima and continues on alone with Mammon and Sakutaro, only to be ambushed by Kasumi and her men, who proceed to beat and taunt her mercilessly, and destroy Maria's diary. Noticing that Kasumi is being manipulated by the spirit of Eva-Beatrice, Ange miraculously summons the Sisters of Purgatory despite the large amount of magical resistance present, killing Kasumi and her men. Before vanishing, Eva-Beatrice resurrects the long-dead, human Eva to shoot and kill Ange, though Mammon destroys her gun, allowing Ange to kill Eva and make peace with her own emotions towards her. Ange meets with Bernkastel, who confirms that she is nothing more than a piece in the game between Battler and Beatrice. However, Ange remains determined to save her family.