Madoka, Yohko's gradmother, senses the return of Tokima: the demon who from the time of the first devil hunter has been the enemy of the Mano family, is approaching. Tokima appears and possesses Ryuichi Asakura, a man who Yohko has a crush on. Tokima tricks Yohko and gets near the clock where Tokima is sealed, but is repelled by special talismans. The next day, Madoka ends up with her past youthful body! Tokima possesses Azusa who takes the clock. Yohko fights Azusa trying to stop her from destroying the clock. Yohko is tricked and "killed". The clock is destroyed and the demon's power released. Yohko is taken to the time of the very first Devil Hunter, Haruka Mano. The two go back into the future and fight against Tokima.

Ayako Mano, who is nearly identical in appearance to Yohko, is introduced; she has spent her life training and honing her abilities so that one day she may defeat Yohko and become the devil hunter of the Mano family, and now she is ready for the final confrontation. Yohko continues to live her life and is unaware of Ayako until she starts stealing away every guy that Yohko has a crush on. Then one night while taking a bath, Yohko hears something outside and gives chase. She then meets up with an Azusa look-alike. She and Ayako attack the unsuspecting Yohko with crushing attacks, while Yohko parries the onslaught. The fight stops, but Ayako vows to finish the fight. Madoka reveals a certain part of her past that relates to the encounter. Yohko's teacher, whom she has a crush on, is used as bait to lure Yohko. Ayako and Yohko meet face-to-face for the showdown. The fight awakens a demon who sleeps deep within the earthand the two must learn to fight together to fend off this powerful creature.