Shinpachi Shimura returns to the Yorozuya and discovers his friends drastically changed during the break. Feeling left behind, he returns home and discovers his sister, Tae Shimura, engaged to Isao Kondo. Tae and Kando enlists Shinpachi into the Shinsengumi and reveals that Tae is pregnant. Shinpachi runs to the ocean and laments the sudden change of those around him. There, he learns about Kyubei Yagyu's sex change to modify her life. They are interrupted by and Kotaro Katsura who also changed his genre due to his Kyubei's characters similarities.

As Katsura and Kyubei fight for the title of the best transgendered, Shinpachi leaves to find that the Shinsengumi members also suffered a sudden change in their selves; Sogo Okita is the leader from the group and orders him and Toshiro Hijikata, Sagaru Yamazaki to attack the Yorozuya. As the three leave, Hijikata knocks out Yamazaki, as he was faking his change. He and Shinpachi learn that an invasion of parasite aliens is responsible for change to their friends and proceed to destroy them. However, they find problems in the outcome, and both Hijikata and and Shinpachi are knocked out in the process, with Gintoki being actually only person without a parasite.