Following Shigeshige's assassination, the new Shogun, Nobunobu begins a purge of the old guard. Matsudaira and Kondo are blamed for failing to prevent the assassination and sentenced to be beheaded and the Shingengumi are disbanded. Lost and without a leader, they must now decide what to do next.

Gin and Hijikata are drinking away their sorrows when Nobunobu and the Mimawarigumi storm the place. Nobunobu, not satisfied with having crushed Shigeshige and thei Shinsengumi attempts to antagonize Hijikata. Suddenly, an unexpected ally arrives.

Whilst Kondo And Katsura attempt to break out of prison, Hijikata must decide which path to walk.

The Shinsengumi and Joi Rebels team up to rescue Kondo and Katsura from the prison island known as Kokujo, but things do not go to plan and they are soon ambushed by a familiar enemy.

Odd Jobs and the Shinsengumi make land on the prison island, but to reach Kondo and Katsura they must content with the Tendoshu and the Mimawarigumi first.

Sasaki Isaburo's past with the Shinsengumi, Matsudaira, and Imai Nobume is finally revealed.

Things come to a head on Kokujo Island, as death itself descends upon the battlefield.

With everyone fighting to regroup and escape, Sasaki makes the same mistake one more time.

The complete fallout of the events at Kokujo Island come to light and brings about farewells nobody thought they'd ever see.