Sirens awaken the No-Star students for the start of “No-Late Day,” where being tardy will get you expelled from Honnouji Academy. With Senketsu in the wash, Ryuko must fight on her own to get through the Discipline Committee’s traps and arrive on time.

Overwhelmed by recent events, Ryuko demands answers from her homeroom teacher, Aikuro Mikisugi. Meanwhile, Uzu Sanageyama, Honnouji Academy’s Athletic Committee Chair and on the Elite Four, makes a request of his own… to challenge Ryuko to a duel!

The Tri-City Schools Raid Trip has commenced! The three-pronged attack on the Kansai region is off to an interesting start, with some unusual defenses to overcome. But what part will Ryuko be able to play, with Senketsu in the hands of the enemy?

Satsuki Kiryuin joins the front line of Osaka to battle Kaneo Takarada, the heir to the Takarada Conglomerate. Using the influence of money, Takarada rallies the locals to fight, but Satsuki is suspicious there is another power behind the city's defense.

Preparations are underway for the Honnouji Academy Cultural & Sports Grand Festival! The school is in a fervor as Ragyo Kiryuin will be attending, but what are her and Satsuki's real intentions for the event?

Ryuko is awake and she's not happy. Intent on killing Ragyo and Nui, she heads out, leaving her allies behind. Without her power, Nudist Beach must decide what their next step is, but a new piece of intel spurs them to action!

The final confrontation is at hand! Will Ragyo and Nui bring on the end of the world, or will Ryuko and the others be able to stop them?

It's graduation at Honnouji Academy, but not everything is as it seems. Ryuko Matoi and the gang must defeat their final enemy.