Seeing Tanjiro try to protect Nezuko even as he collapses triggers memories of Rui’s own past. Once a sickly boy, he became a demon after meeting Muzan Kibutsuji who gave him a share of his blood. But after he turns into a demon, his own parents try to kill him. Ever since then, he has been searching for a family. Seeking a bond that he can never hope to acquire...

Tanjiro, who's undergoing rehabilitation training at the Butterfly Mansion, works hard to keep up his Total Concentration Breathing techniques all day long. Though at first, he's helpless against Kanao, a swordswoman in his class who's training him, he slowly but surely begins to make some headway. Seeing Tanjiro devote himself to training every single day, Zenitsu and Inosuke resume training as well, but...

As Tanjiro and the others are devoted to their rehabilitation training, the absolute master of the demons, Muzan Kibutsuji, gathers the Lower Ranks of the Twelve Kizuki. The demons, too, are preparing to make their next move. As training draws to a close, Tanjiro has improved to the point where he can fight on equal terms with Kanao. Just then, he receives word of a new mission from his Kasugai Crow.

After the Mugen Train incident, Akaza visits Muzan Kibutsuji. Guided by a crow, Tanjiro Kamado heads off to the Rengoku residence to convey Kyojuro Rengoku's last messages to his father and little brother. Tanjiro's group return to the Butterfly Mansion.

An Upper Rank Demon has been defeated for the first time in a hundred years by Uzui, Tanjiro, and his comrades' effort. This news causes a ripple effect not only among the members of the Demon Slayer Corps, but also the Upper Rank Demons, who have been summoned by Muzan Kibutsuji. Meanwhile, Tanjiro is recovering at the Butterfly Mansion when he discovers that he has upset his swordsmith Haganezuka after chipping his sword and decides to head to the village where the swordsmiths live to talk to him in person.

The Hashira, Sanemi and Iguro, along with their comrades head to an abandoned castle on top of a mountain for a mission, while Tanjiro is being treated at the Butterfly Mansion for the wounds he suffered at the Swordsmith Village. Later on, the Hashira gather at the Ubuyashiki Mansion to hold a Hashira meeting, where Muichiro reveals how the marks manifested. In an effort to strengthen the entire Demon Slayer Corps from the bottom up, the Hashira Training begins...

Kagaya's Kasugai Crow suddenly appears in front of Tamayo and invites her to the Demon Slayer headquarters — even though she is a demon. Meanwhile, after receiving a letter from Kagaya at the Butterfly Mansion, Tanjiro pays a visit to Giyu, who is not participating in the Hashira Training. At first, Tanjiro is rejected by Giyu but patiently continues to try to talk to him. Giyu, who gives in to Tanjiro's persistence, begins to share the reason why he won't participate in the training.

During a silent night when the moon shines, Kagaya finally meets Muzan Kibutsuji, who appears at the Ubuyashiki Mansion.