Minions er gule, små hjelpere som har eksistert siden tidenes morgen. De har utviklet seg fra gule encellede organismer til små vesener med ett hovedmål: Å hjelpe verdenshistoriens verste skurker. Men etter hvert som det ble færre skurker i verden, trakk de til seg tilbake til Antarktis for å skape seg et helt nytt liv der. På midten av 60-tallet rådte det stor en stor minionsdepresjon, så noen modige minions dro ut i verden for å finne en ny sjefsskurk. De finner veien til en skurkekongress, hvor de konkurrerer om å få bli de nye hjelperne til Scarlet Overkill - en stilfull og ambisiøs skurk som ønsker å ta over verden og bli tidenes første kvinnelige superskurk.

Superhunden Krypto må overbevise en merkelig gruppe herreløse dyr – en hund, en gris, en skilpadde og et ekorn – for å mestre de nyvunne kreftene og hjelpe ham med å redde Justice League.

It is written among the limitless constellations of the celestial heavens, and in the depths of the emerald seas, and upon every grain of sand in the vast deserts, that the world which we see is an outward and visible dream, of an inward and invisible reality ... Once upon a time there was a golden city. In the center of the golden city, atop the tallest minaret, were three golden balls. The ancients had prophesied that if the three golden balls were ever taken away, harmony would yield to discord, and the city would fall to destruction and death. But... the mystics had also foretold that the city might be saved by the simplest soul with the smallest and simplest of things. In the city there dwelt a lowly shoemaker, who was known as Tack the Cobbler. Also in the city... existed a Thief, who shall be... nameless.

Mantis Commander and his minions try their best to be the bad guys they think they are.

After being cast into the shadows, the villainous organization known as "Limbo" rises from the darkness. Led by the scourge Doomfist, they seek to bring about a world of change. But a rag-tag group of heroes bands together to oppose the darkness.