故事敘述主角的哥哥林肯·布魯斯（Lincoln Burrows）被誣陷殺害副總統之弟，被判處死刑並將於不久後執行。麥可·史考菲（Michael Scofield）得知其兄的冤情後，秘密策劃了一套詳盡可行的計劃，欲幫助林肯出獄。誰知道一連串陰謀不斷發生在他們及其身邊的人的身上。

Through globalization, many countries have been opened and barriers removed to ensure easy trade, travel and cultural diversity. However, this openness has given opportunities to criminals looking to exploit the system and ultimately threaten our global safety. As Europe has become a "safe house" for criminals eluding law enforcers, a special kind of law enforcement team is needed to handle specific ongoing crimes on a global level. "Crossing Lines" is the story of one such team, made up of five international cops, headed by Captain Daniel. The team - comprised of individuals who have little in common - must learn to live and work under the most dangerous and potentially deadly conditions. Housed in an unused storage section underneath the ICC, this mismatched team faces bureaucratic, jurisdictional and cultural obstacles while traversing continents in pursuit of justice.