Aspiring super-villain Dr. Horrible wants to join the Evil League of Evil and win the girl of his dreams, but his nemesis, Captain Hammer, stops him at every turn.

Neon, a lonely girl working the late-night shift at a laundromat, carelessly thieves a briefcase filled with a million in cash from her crush Night's apartment. Night is a murder mystery novelist who came to Neon's rescue at the laundromat. When he catches on that she's the thief and comes to get the bag back, it's already gone missing. The two have to team up to search for the true thief with only one clue, the laundromat's service log. In the record are: Judo, a good-looking university student who came to study; P'Chompoo, Night's landlord; Momay, a sexy karaoke girl; and Nick and Smile, a bitchin' rock couple.