Yohko Mano is introduced. Yohko goes about her daily life; day dreaming about a cute guy named Hideki. Yohko learns that her family has a long history of being devil hunters. But this time the demons decide to take out the next devil hunter before she can fight back. After a failed attempt to take Yohko's virginity (so that she can't become a devil hunter), the demons decide to use Hideki as bait. Yohko must become the 108th devil hunter and rescue the man of her dreams.

Yohko trains to become a good devil hunter. Nearby a local construction site destroys a forest in which sleeping spirits lie. The spirits are disturbed by this and start harassing the construction workers. Azusa Kanzaki, a Devil Hunter in training, is introduced. Azusa came to become an apprentice to Yohko. The two team up to fight against the spirits that are harassing the workers.

Yohko has a dream about a man named Master Biryu. Yohko is transported to another dimension and learns that Biryu has been imprisoned. Yohko learns that she must save Master Biryu (although she develops a crush on him). Meanwhile, Azusa saw Yohko disappear and attempts to get Yohko back with Yohko's friend, Chi. They manage to get to where Yohko is, but fail and manage to get themselves back where the started. She must fight the demon holding Master Biryu captive in order to free him.

A tribute to Devil Hunter Yohko, with a collection of music videos. These videos feature songs from the series(including three new songs), put to scenes from the anime, colored manga picture, an original animated video featuring chibified versions of Yohko and Azusa, and two live videos.

Ayako Mano, who is nearly identical in appearance to Yohko, is introduced; she has spent her life training and honing her abilities so that one day she may defeat Yohko and become the devil hunter of the Mano family, and now she is ready for the final confrontation. Yohko continues to live her life and is unaware of Ayako until she starts stealing away every guy that Yohko has a crush on. Then one night while taking a bath, Yohko hears something outside and gives chase. She then meets up with an Azusa look-alike. She and Ayako attack the unsuspecting Yohko with crushing attacks, while Yohko parries the onslaught. The fight stops, but Ayako vows to finish the fight. Madoka reveals a certain part of her past that relates to the encounter. Yohko's teacher, whom she has a crush on, is used as bait to lure Yohko. Ayako and Yohko meet face-to-face for the showdown. The fight awakens a demon who sleeps deep within the earthand the two must learn to fight together to fend off this powerful creature.