After the Mugen Train incident, Akaza visits Muzan Kibutsuji. Guided by a crow, Tanjiro Kamado heads off to the Rengoku residence to convey Kyojuro Rengoku's last messages to his father and little brother. Tanjiro's group return to the Butterfly Mansion.

After Tanjiro visits the Rengoku residence and meets Kyojuro's younger brother, Senjuro, to deliver Kyojuro's last message, Tanjiro spends his days training and completing missions. One day, he returns to the Butterfly Mansion to find Sound Hashira, Tengen Uzui, attempting to take Aoi and Naho away on a mission against their will. Tanjiro, Zenitsu and Inosuke offer to go on that mission instead, and they head to their next destination - the Entertainment District.

After arriving at the Entertainment District, Tanjiro and his comrades infiltrate each House where Uzui's wives, Suma, Makio and Hinatsuru, work. While working as apprentices and searching for their whereabouts, Inosuke hears that Makio has been shutting herself away from everyone in her room.

As Tanjiro close in on the demon that has been preying upon the entertainment district. But even as he closes in, the others continue to follow their leads toward their query. Who will find the demon first? And will they be able to survive the confrontation when they do?

After being told of Zenitsu’s disappearance and to leave the Entertainment District by Uzui, Tanjiro and Inosuke decide that they will rescue Zenitsu. That evening, Tanjiro picks up a scent of a demon, which leads him to Koinatsu Oiran being swallowed by Daki’s belt. With Uzui nowhere in sight, Tanjiro must face the Upper Six demon alone.

During the fierce battle with Daki, Tanjiro continues to unleash the Hinokami Kagura, which causes a huge strain on his body. Meanwhile, Inosuke reaches the Belt Demon’s nest and saves people who have been captured. Makio, Suma, and Zenitsu, who have been freed from the belt, join in on the fight. As the ceiling of the nest is broken, Uzui appears wielding his swords.

Enraged by Daki's contempt for human life, Tanjiro attacks her with more intensity and gradually corners the demon. But he's already gone past his physical limit. As Tanjiro collapses and all seems lost again, Nezuko appears. A vicious battle ensues and Nezuko starts transforming into a more demon-like form...

After Nezuko attempts to attack innocent bystanders as her demon form advances, Tanjiro is finally able to make her fall asleep by singing a lullaby. Meanwhile, another demon emerges out of Daki's body after being beheaded by Uzui - Gyutaro, Daki's older brother and the other form of Upper Six. Uzui must now face both demons, Daki and Gyutaro.

Tanjiro and Uzui have been separated by Gyutaro’s Blood Demon Art. As Tanjiro joins the struggling Zenitsu and Inosuke, they manage to decapitate Daki at last thanks to their coordinated barrage of sword strikes. However, when Inosuke tries to run off with Daki’s head, he gets pierced by Gyutaro’s scythe. Uzui has also collapsed, leaving Tanjiro and the others with their backs to the wall.

Uzui deflects all of Gyutaro's Blood Demon Art attacks, and despite getting pierced in the jaw, Tanjiro is able to slice off Gyutaro's head. Though wounded from head to toe, Zenitsu and Inosuke also behead Daki, and they succeed in decapitating both demons at the same time. Just when it seems as though they've defeated the Upper Six demons after a fierce struggle, Gyutaro unleashes his Blood Demon Art...